What this CURE is about:
Students will build a library of protein variants that will be aimed at assessing the binding interaction between two human proteins, in which the interaction has been implicated in ferroptosis – a non-apoptotic form of programmed cell death. Students will pick an amino acid residue (implicated in binding) to mutate, design primers, carry out mutagenesis, sequence mutant DNA, and optimize expression and purification of the mutant protein.