Just Accepted
2023
42. Luis Guevara, Melissa Gouge, Amanda Ohler, S. Gage Hill, Soham Patel, and Adam R. Offenbacher. Effect of Solvent Viscosity on the Activation Barrier of Hydrogen Tunneling in the Lipoxygenase Reaction. Arch. Biochem. Biophys. 2023, 747, 109740.
41. Denis L. Rohlik, Ethan Patel, Nathaniel C. Gilbert, Adam R. Offenbacher, and Brandon L. Garcia. Investigating membrane-binding properties of lipoxygenases using surface plasmon resonance. Biochem. Biophys Res. Commun. 2023, 670, 47-54.
40. Ajay Sharma, Chris Whittington, Mohammed Jabed, S. Gage Hill, Anastasiia Kostenko, Tao Yu, Pengfei Li, Peter E. Doan, Brian M. Hoffman, and Adam R. Offenbacher. 13C Electron Nuclear Double Resonance Spectroscopy-Guided Molecular Dynamics Computations Reveal the Structure of the Enzyme-Substrate Complex of an Active, N-linked Glycosylated Lipoxygenase. Biochemistry 2023, 62, 1531-1543.
39. Paulo Zaragoza*, Adam Offenbacher*, Shenshen Hu, Christine Gee, Zachary Firestein, Natalie Minnetian, Zhenyu Deng, FLora Fan, Anthony Iavarone, and Judith Klinman. Temporal and Spatial Resolution of Distal Protein Motions that Activate Hydrogen Tunneling in Soybean Lipoxygenase. Proc. Natl. Acad. Sci. USA 2023, 120, e2211630120.
38. Jose Pinelo, Pragya Manandhar, Grega Popovic, Katherine Ray, Mehmet Tasdelen, Quoc Nguyen, Anthony Iavarone, Adam Offenbacher, Nathan Hudson, and Mehmet Sen. Systematic mapping of the conformational landscape and dynamism of soluble fibrinogen. J. Thromb. Haemost. 2023, 21, 1529-1543.
37. Kristin Tyson, Chanin Tangtartharakul, Matthias Zeug, Nathan Findling, Alice Haddy, Eli Hvastkovs, Jun-yong Choe, Judy Kim, and Adam Offenbacher. Electrochemical and structural study of the buried tryptophan in azurin: effects of hydration and polarity of the redox potential of W48. J. Phys. Chem. B 2023, 127, 133-143.
36. Michelle Tran, Rachel Signorelli, Adriana Yamaguchi, Eefei Chen, Michael Holinstat, Anthony Iavarone, Adam Offenbacher, and Ted Holman. Biochemical and hydrogen-deuterium exchange studies of the single nucleotide polymorphism Y649C in human platelet 12-lipoxygenase linked to a bleeding disorder. Arch. Biochem. Biophys. 2023, 733, 109472.
2022
35. Courtney Labrecque, Aubree Nolan, Angela Develin, Abdul Castillo, Adam Offenbacher, and Brian Fuglestad. Membrane-mimicking reverse micelles for high-resolution interfacial study of proteins and membranes. Langmuir 2022, 38, 3676-3686.
34. Grega Popovic, Nicholas Kirby, Taylor Dement, Kristine Peterson, Caroline Daub, Heather Belcher, Martin Guthold, Adam R. Offenbacher, and Nathan Hudson. Development of Transient Recombinant Expression and Affinity Chromatography Systems for Human Fibrinogen. Int. J. Mol. Sci. 2022, 23, 1054.
33. Daniella Roberts, Amy Benton, Claire Fabian-Bayola, Anne Spuches, and Adam R. Offenbacher. Thermodynamic and Biophysical Study of Fatty Acid Effector Binding to Soybean Lipoxygenase: Implications for Allostery Driven by Helix alpha2 Dynamics. FEBS Lett. 2022, 596, 350-359.
2021
32. Mohammad S. Safiarian, R. Atlee Watson, Raquel L. Lieberman, Bridgette A. Barry, and Adam R. Offenbacher. E. coli Ribonucleotide Reductase b2 Subunit Inactivation by Triapine Occurs through Binding of a Triapine-Fe(II) Adduct. J. Phys. Chem. Lett. 2021, 12, 9020-9025.
31. Wan-Chen Tsai, Nathan C. Gilbert, Amanda Ohler, Michelle Armstrong, Steven Perry, Chakrapani Kalyanaraman, Adam Yasgar, Ganesha Rai, Anton Simeonov, Ajit Jadhav, Melissa Standley, Hsiau-Wei Lee, Phillip Crews, Anthony Iavarone, Matthew Jacobson, David Neau, Adam R. Offenbacher, Marcia Newcomer, Theodore R. Holman. Kinetic and Structural Investigations of Novel Inhibitors of Human Epithelial 15-Lipoxygenase-2. Bioorgan. Med. Chem. 2021, 46, 116349.
30. R. Atlee Watson, Adam R. Offenbacher, and Bridgette A. Barry. Detection of Catalytically Linked Conformational Changes in Wildtype Class Ia Ribonucleotide Reductase using Reaction-Induced FTIR Spectroscopy. J. Phys. Chem. B 2021, 125, 8362-8372.
29. Wan-Chen Tsai, Ansari Mukhtar Aleem, Chris Whittington, Wilian A. Cortopassi, Chakrapani Kalyanaraman, Angel Baroz, Anthony T. Iavarone, Ewa Skrzypczak-Jankun, Matthew P. Jacobson, Adam R. Offenbacher and Theodore Holman. Mutagenesis, Hydrogen−Deuterium Exchange, and Molecular Dynamics Investigations Establish the Dimeric Interface of Human Platelet-Type 12-Lipoxygenase. Biochemistry. 2021, 60, 802-812.
28. Amanda Ohler, Hanna Long, Kei Ohgo, Kristin Tyson, David Murray, Amanda Davis, Chris Whittington, Eli G. Hvastkovs, Liam Duffy, Alice Haddy, Andrew L. Sargent, William E. Allen and Adam R. Offenbacher. Synthesis of redox-active fluorinated 5-hydroxytryptophans as molecular reporters for biological electron transfer. ChemComm. 2021, 57, 3107-3110.
2020
27. Adam R. Offenbacher, Justine P. Roth, and Judith P. Klinman. Kinetic isotope effects. Encyclopedia of Biochemistry 3rd Edition. (book chapter)
26. Chris Whittington, John Latham, and Adam R. Offenbacher. Tunneling through the barriers: resolving the origins of the activation of C-H bonds catalyzed by enzymes. ACS Symposium Series “Bridging Structure and Function in Mechanistic Enzymology”. 2020, Chapter 7, 139-160.
25. Adam R. Offenbacher and Theodore R. Holman. Fatty acid allosteric regulation of C-H activation in plant and animal lipoxygenases. Molecules. 2020, 25, 3374.
24. Kristin J. Tyson, Amanda N. Davis, Jessica L. Norris, Libero J. Bartolotti, Eli G. Hvastkovs, and Adam R. Offenbacher. Impact of local electrostatics on the redox properties of tryptophan radicals in azurin: implications for redox-active tryptophans in proton-coupled electron transfer. J. Phys. Chem. Letters, 2020, 11, 2408-2413.
23. Adam R. Offenbacher, Ajay Sharma, Peter Doan, Judith P. Klinman, and Brian M. Hoffman. The soybean lipoxygenase−substrate complex: correlation between the properties of tunneling-ready states and ENDOR detected structures of ground states. Biochemistry, 2020, 59, 901-910.
22. Adam R. Offenbacher and Bridgette A. Barry. A proton wire mediates proton-coupled electron transfer from hydroxyurea and other hydroxamic acids to tyrosyl radical in class Ia ribonucleotide reductase. J. Phys. Chem. B, 2020, 124, 345-354.
2019
21. Shenshen Hu, Adam R. Offenbacher, Edbert D. Lu, and Judith P. Klinman. Comparative kinetic isotope effects on first- and second-order rate constants of soybean lipoxygenase variants uncover a substrate-binding network. J. Biol. Chem. 2019, 294, 18069-18076.
20. Jan Paulo Zaragoza, Andy Nguy, Natalie Minnetian, Zhenyu Deng, Anthony T. Iavarone, Adam R. Offenbacher, and Judith P. Klinman. Detecting and Characterizing the Kinetic Activation of Thermal Networks in Proteins: Thermal Transfer from a Distal, Solvent Exposed Loop to the Active Site in Soybean Lipoxygenase. J. Phys. Chem. B 2019, 123, 8662-8674.
19. Anastasiia Kostenko, Katherine Ray, Anthony T. Iavarone, and Adam R. Offenbacher. Kinetic Characterization of the C-H Activation Step for the Lipoxygenase from the Pathogenic Fungus Magnaporthe oryzae: Impact of N-linked Glycosylation. Boichemistry 2019, 58, 3193-3203.
18. Shenshen Hu, Adam R. Offenbacher, Erin M. Thompson, Christine L. Gee, Jarett Wilcoxen, Cody A. M. Carr, Daniil M. Prigozhin, Vanessa Yang, Tom Alber, R. David Britt, James S. Fraser, and Judith P. Klinman. Biophysical Characterization of a Disabled Double Mutant of Soybean Lipoxygenase: The “Undoing” of Precise Substrate Positioning Relative to Metal Cofactor and an Identified Dynamical
Network. J. Am. Chem. Soc. 2019, 141, 1555-1567.
2018
17, Judith P. Klinman and Adam R. Offenbacher. Understanding biological hydrogen transfer through the lens of temperature dependent kinetic isotope effects. Acc. Chem. Res. 2018, 51, 1966–1974
16. Adam R. Offenbacher, Anthony T. Iavarone, and Judith P. Klinman. Hydrogen-deuterium exchange reveals long-range dynamical allostery in soybean lipoxygenase. J. Biol. Chem. 2018, 293, 1138-1148.
Prior Publications:
2017
15. Judith P. Klinman, Adam R. Offenbacher, and Shenshen Hu. The Origins of Enzyme Catalysis: Experimental Findings for C-H Activation, New Models and Their Relevance to Prevailing Theoretical Constructs. J. Am. Chem. Soc. (Perspective) 2017, 139, 18409-18427.
14. Adam R. Offenbacher, Shenshen Hu, Erin M. Poss, Cody A. M. Carr, Alexander D. Scouras, Daniil M. Prigozhin, Anthony T. Iavarone, Ali Palla, Tom Alber, James S. Fraser, and Judith P. Klinman. Hydrogen deuterium exchange of lipoxygenase uncovers a relationship between distal, solvent exposed protein motions and the thermal activation barrier for catalytic proton coupled electron tunneling. ACS Cent. Sci. 2017, 3, 570-579.
13. Masaki Horitani*, Adam R. Offenbacher*, Cody A. M. Carr, Tao Yu, Sharon Hammes-Schiffer, Judith P. Klinman, and Brian M. Hoffman. Active site structure of a lipoxygenase-substrate complex from 13C ENDOR spectroscopy reveals structural basis for tunneling properties that underlie C-H activation. J. Am. Chem. Soc. 2017, 139, 1984-1997.
2016
12. Adam R. Offenbacher, Hui Zhu, and Judith P. Klinman. Synthesis of site-specifically 13C labeled linoleic acids. Tetrahedron Lett. 2016, 57, 4537-4540.
2014
11. Adam R. Offenbacher, Cynthia V. Pagba, Brandon C. Polander, Udita Brahmachari, and Bridgette A. Barry. The first site-specific incorporation of a non-canonical amino acid into the photosynthetic oxygen-evolving complex. ACS Chem. Biol. 2014, 9, 891-896.
10. Adam R. Offenbacher, R. Atlee Watson, Cynthia V. Pagba, and Bridgette A. Barry. Redox-dependent structural coupling between the a2 and β2 subunits in E. coli ribonucleotide reductase. J. Phys. Chem. B 2014, 118, 2993–3004.
2013
9. Adam R. Offenbacher, Brandon C. Polander, and Bridgette A. Barry. An intrinsically disordered photosystem II subunit, PsbO, provides a structural template and a sensor of the hydrogen-bonding network in photosynthetic water oxidation. J. Biol. Chem. 2013, 288, 29056-29068.
8. Adam R. Offenbacher, Ellen C. Minnihan, JoAnne Stubbe, and Bridgette A. Barry. Redox-linked changes to the hydrogen bonding network of ribonucleotide reductase β2. J. Am. Chem. Soc. 2013, 135, 6380–6383.
7. Adam R. Offenbacher, Lori A. Burns, C. David Sherrill, and Bridgette A. Barry. Redox-linked conformational control of proton-coupled electron transfer: Y122 in the ribonucleotide reductase β2 subunit. J. Phys. Chem. B 2013, 117, 8457–8468.
2012
6. Bridgette A. Barry, Jun Chen, James Keough, David Jenson, Adam Offenbacher, and Cynthia Pagba. Proton-coupled electron transfer and redox-active tyrosines: Structure and function of the tyrosyl radicals in ribonucleotide reductase and photosystem II. J. Phys. Chem. Lett. 2012, 3, 543-554.
2011
5. Adam R. Offenbacher, Jun Chen, and Bridgette A. Barry. Perturbations of aromatic amino acids areassociated with iron cluster assembly in ribonucleotide reductase. J. Am. Chem. Soc. 2011, 133, 6978-6988.
2009
4. Nicholas J. Dunn, William H. Humphries, IV, Adam R. Offenbacher, Travis L. King, and Jeffrey A. Gray. pH-dependent cis –> trans isomerization rates for azobenzene dyes in aqueous solution. J. Phys.Chem. A 2009, 113, 13144–13151.
3. A. R. Offenbacher, I. R. Vassiliev, M. R. Seyedsayamdost, J. Stubbe, and B. A. Barry. Redox-linked structural changes in ribonucleotide reductase. J. Am. Chem. Soc. 2009, 131, 7496–7497.
2. Adam Offenbacher, Kimberley N. White, Indranil Sen, Allen G. Oliver, Joseph P. Konopelski,Bridgette A. Barry, and Ólöf Einarsdóttir. A spectroscopic investigation of a tridentate Cu-complex mimicking the tyrosine-histidine cross-link of cytochrome c oxidase. J. Phys. Chem. B 2009, 113, 7407–7417.
2005
1. Ilya R. Vassiliev, Adam R. Offenbacher, and Bridgette A. Barry. Redox-active tyrosine residues inpentapeptides. J. Phys. Chem. B 2005, 109, 23077–23085